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Fig. 4. (A) Real-time RT-PCR analysis of differentially expressed GABAAa-1 gene in spinal dorsal horns (L6–S1) from rats with neonatal cystitis. Two different treatment protocols were followed. Tissues were examined <t>on</t> <t>P60</t> from the unchallenged protocol and on P30 from the re-challenged protocol. The ordinate value 2DCT corresponds to the mRNA expression relative to reference gene ribosomal <t>RNA</t> (rRNA); data are represented as mean ± SD (n = 5 per group). (B) Western blot analysis of GABAAa-1 protein in spinal dorsal horn samples (L6–S1) from rats with neonatal cystitis. The intensity of GABAAa-1 immunoreactivity for different tissues was normalized against the intensity of b-actin expression for the same tissue. Results are expressed as means ± SD (n = 5 per group). ⁄Significant difference at p < 0.05. (C) GABAAa-1 immunostaining of differentially expressed GABAAa-1 gene in spinal dorsal horns (L6–S1) from rats with neonatal cystitis. The tissues were examined on P60 after zymosan/saline treatment at P14 to P16. The scale bar is 50 lm. The intensity of staining for 10 individual cells from each group was determined; data are presented as means ± SD. ⁄Significant difference at p < 0.001.
Rna Extraction Kit, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Fig. 4. (A) Real-time RT-PCR analysis of differentially expressed GABAAa-1 gene in spinal dorsal horns (L6–S1) from rats with neonatal cystitis. Two different treatment protocols were followed. Tissues were examined <t>on</t> <t>P60</t> from the unchallenged protocol and on P30 from the re-challenged protocol. The ordinate value 2DCT corresponds to the mRNA expression relative to reference gene ribosomal <t>RNA</t> (rRNA); data are represented as mean ± SD (n = 5 per group). (B) Western blot analysis of GABAAa-1 protein in spinal dorsal horn samples (L6–S1) from rats with neonatal cystitis. The intensity of GABAAa-1 immunoreactivity for different tissues was normalized against the intensity of b-actin expression for the same tissue. Results are expressed as means ± SD (n = 5 per group). ⁄Significant difference at p < 0.05. (C) GABAAa-1 immunostaining of differentially expressed GABAAa-1 gene in spinal dorsal horns (L6–S1) from rats with neonatal cystitis. The tissues were examined on P60 after zymosan/saline treatment at P14 to P16. The scale bar is 50 lm. The intensity of staining for 10 individual cells from each group was determined; data are presented as means ± SD. ⁄Significant difference at p < 0.001.
Rneasy Fibrous Tissue Kit, supplied by Qiagen, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Fig. 4. (A) Real-time RT-PCR analysis of differentially expressed GABAAa-1 gene in spinal dorsal horns (L6–S1) from rats with neonatal cystitis. Two different treatment protocols were followed. Tissues were examined <t>on</t> <t>P60</t> from the unchallenged protocol and on P30 from the re-challenged protocol. The ordinate value 2DCT corresponds to the mRNA expression relative to reference gene ribosomal <t>RNA</t> (rRNA); data are represented as mean ± SD (n = 5 per group). (B) Western blot analysis of GABAAa-1 protein in spinal dorsal horn samples (L6–S1) from rats with neonatal cystitis. The intensity of GABAAa-1 immunoreactivity for different tissues was normalized against the intensity of b-actin expression for the same tissue. Results are expressed as means ± SD (n = 5 per group). ⁄Significant difference at p < 0.05. (C) GABAAa-1 immunostaining of differentially expressed GABAAa-1 gene in spinal dorsal horns (L6–S1) from rats with neonatal cystitis. The tissues were examined on P60 after zymosan/saline treatment at P14 to P16. The scale bar is 50 lm. The intensity of staining for 10 individual cells from each group was determined; data are presented as means ± SD. ⁄Significant difference at p < 0.001.
Rneasy Fibrous Tissue Midi Kit, supplied by Qiagen, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Fig. 4. (A) Real-time RT-PCR analysis of differentially expressed GABAAa-1 gene in spinal dorsal horns (L6–S1) from rats with neonatal cystitis. Two different treatment protocols were followed. Tissues were examined <t>on</t> <t>P60</t> from the unchallenged protocol and on P30 from the re-challenged protocol. The ordinate value 2DCT corresponds to the mRNA expression relative to reference gene ribosomal <t>RNA</t> (rRNA); data are represented as mean ± SD (n = 5 per group). (B) Western blot analysis of GABAAa-1 protein in spinal dorsal horn samples (L6–S1) from rats with neonatal cystitis. The intensity of GABAAa-1 immunoreactivity for different tissues was normalized against the intensity of b-actin expression for the same tissue. Results are expressed as means ± SD (n = 5 per group). ⁄Significant difference at p < 0.05. (C) GABAAa-1 immunostaining of differentially expressed GABAAa-1 gene in spinal dorsal horns (L6–S1) from rats with neonatal cystitis. The tissues were examined on P60 after zymosan/saline treatment at P14 to P16. The scale bar is 50 lm. The intensity of staining for 10 individual cells from each group was determined; data are presented as means ± SD. ⁄Significant difference at p < 0.001.
Rna Fibrous Tissue Kit, supplied by Qiagen, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Fig. 4. (A) Real-time RT-PCR analysis of differentially expressed GABAAa-1 gene in spinal dorsal horns (L6–S1) from rats with neonatal cystitis. Two different treatment protocols were followed. Tissues were examined <t>on</t> <t>P60</t> from the unchallenged protocol and on P30 from the re-challenged protocol. The ordinate value 2DCT corresponds to the mRNA expression relative to reference gene ribosomal <t>RNA</t> (rRNA); data are represented as mean ± SD (n = 5 per group). (B) Western blot analysis of GABAAa-1 protein in spinal dorsal horn samples (L6–S1) from rats with neonatal cystitis. The intensity of GABAAa-1 immunoreactivity for different tissues was normalized against the intensity of b-actin expression for the same tissue. Results are expressed as means ± SD (n = 5 per group). ⁄Significant difference at p < 0.05. (C) GABAAa-1 immunostaining of differentially expressed GABAAa-1 gene in spinal dorsal horns (L6–S1) from rats with neonatal cystitis. The tissues were examined on P60 after zymosan/saline treatment at P14 to P16. The scale bar is 50 lm. The intensity of staining for 10 individual cells from each group was determined; data are presented as means ± SD. ⁄Significant difference at p < 0.001.
Qiamprneasy Fibrous Tissue Mini Kit, supplied by Qiagen, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Fig. 4. (A) Real-time RT-PCR analysis of differentially expressed GABAAa-1 gene in spinal dorsal horns (L6–S1) from rats with neonatal cystitis. Two different treatment protocols were followed. Tissues were examined <t>on</t> <t>P60</t> from the unchallenged protocol and on P30 from the re-challenged protocol. The ordinate value 2DCT corresponds to the mRNA expression relative to reference gene ribosomal <t>RNA</t> (rRNA); data are represented as mean ± SD (n = 5 per group). (B) Western blot analysis of GABAAa-1 protein in spinal dorsal horn samples (L6–S1) from rats with neonatal cystitis. The intensity of GABAAa-1 immunoreactivity for different tissues was normalized against the intensity of b-actin expression for the same tissue. Results are expressed as means ± SD (n = 5 per group). ⁄Significant difference at p < 0.05. (C) GABAAa-1 immunostaining of differentially expressed GABAAa-1 gene in spinal dorsal horns (L6–S1) from rats with neonatal cystitis. The tissues were examined on P60 after zymosan/saline treatment at P14 to P16. The scale bar is 50 lm. The intensity of staining for 10 individual cells from each group was determined; data are presented as means ± SD. ⁄Significant difference at p < 0.001.
Rneasy Fibrous Tissue Assay Kit, supplied by Qiagen, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Fig. 4. (A) Real-time RT-PCR analysis of differentially expressed GABAAa-1 gene in spinal dorsal horns (L6–S1) from rats with neonatal cystitis. Two different treatment protocols were followed. Tissues were examined <t>on</t> <t>P60</t> from the unchallenged protocol and on P30 from the re-challenged protocol. The ordinate value 2DCT corresponds to the mRNA expression relative to reference gene ribosomal <t>RNA</t> (rRNA); data are represented as mean ± SD (n = 5 per group). (B) Western blot analysis of GABAAa-1 protein in spinal dorsal horn samples (L6–S1) from rats with neonatal cystitis. The intensity of GABAAa-1 immunoreactivity for different tissues was normalized against the intensity of b-actin expression for the same tissue. Results are expressed as means ± SD (n = 5 per group). ⁄Significant difference at p < 0.05. (C) GABAAa-1 immunostaining of differentially expressed GABAAa-1 gene in spinal dorsal horns (L6–S1) from rats with neonatal cystitis. The tissues were examined on P60 after zymosan/saline treatment at P14 to P16. The scale bar is 50 lm. The intensity of staining for 10 individual cells from each group was determined; data are presented as means ± SD. ⁄Significant difference at p < 0.001.
Rna Fibrous Mini Kit, supplied by Qiagen, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Fig. 4. (A) Real-time RT-PCR analysis of differentially expressed GABAAa-1 gene in spinal dorsal horns (L6–S1) from rats with neonatal cystitis. Two different treatment protocols were followed. Tissues were examined <t>on</t> <t>P60</t> from the unchallenged protocol and on P30 from the re-challenged protocol. The ordinate value 2DCT corresponds to the mRNA expression relative to reference gene ribosomal <t>RNA</t> (rRNA); data are represented as mean ± SD (n = 5 per group). (B) Western blot analysis of GABAAa-1 protein in spinal dorsal horn samples (L6–S1) from rats with neonatal cystitis. The intensity of GABAAa-1 immunoreactivity for different tissues was normalized against the intensity of b-actin expression for the same tissue. Results are expressed as means ± SD (n = 5 per group). ⁄Significant difference at p < 0.05. (C) GABAAa-1 immunostaining of differentially expressed GABAAa-1 gene in spinal dorsal horns (L6–S1) from rats with neonatal cystitis. The tissues were examined on P60 after zymosan/saline treatment at P14 to P16. The scale bar is 50 lm. The intensity of staining for 10 individual cells from each group was determined; data are presented as means ± SD. ⁄Significant difference at p < 0.001.
Rneasy Mini Rna Fibrous Tissue Kit, supplied by Qiagen, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Fig. 4. (A) Real-time RT-PCR analysis of differentially expressed GABAAa-1 gene in spinal dorsal horns (L6–S1) from rats with neonatal cystitis. Two different treatment protocols were followed. Tissues were examined <t>on</t> <t>P60</t> from the unchallenged protocol and on P30 from the re-challenged protocol. The ordinate value 2DCT corresponds to the mRNA expression relative to reference gene ribosomal <t>RNA</t> (rRNA); data are represented as mean ± SD (n = 5 per group). (B) Western blot analysis of GABAAa-1 protein in spinal dorsal horn samples (L6–S1) from rats with neonatal cystitis. The intensity of GABAAa-1 immunoreactivity for different tissues was normalized against the intensity of b-actin expression for the same tissue. Results are expressed as means ± SD (n = 5 per group). ⁄Significant difference at p < 0.05. (C) GABAAa-1 immunostaining of differentially expressed GABAAa-1 gene in spinal dorsal horns (L6–S1) from rats with neonatal cystitis. The tissues were examined on P60 after zymosan/saline treatment at P14 to P16. The scale bar is 50 lm. The intensity of staining for 10 individual cells from each group was determined; data are presented as means ± SD. ⁄Significant difference at p < 0.001.
Fibrous Tissue Mini Kit, supplied by Qiagen, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Fig. 4. (A) Real-time RT-PCR analysis of differentially expressed GABAAa-1 gene in spinal dorsal horns (L6–S1) from rats with neonatal cystitis. Two different treatment protocols were followed. Tissues were examined <t>on</t> <t>P60</t> from the unchallenged protocol and on P30 from the re-challenged protocol. The ordinate value 2DCT corresponds to the mRNA expression relative to reference gene ribosomal <t>RNA</t> (rRNA); data are represented as mean ± SD (n = 5 per group). (B) Western blot analysis of GABAAa-1 protein in spinal dorsal horn samples (L6–S1) from rats with neonatal cystitis. The intensity of GABAAa-1 immunoreactivity for different tissues was normalized against the intensity of b-actin expression for the same tissue. Results are expressed as means ± SD (n = 5 per group). ⁄Significant difference at p < 0.05. (C) GABAAa-1 immunostaining of differentially expressed GABAAa-1 gene in spinal dorsal horns (L6–S1) from rats with neonatal cystitis. The tissues were examined on P60 after zymosan/saline treatment at P14 to P16. The scale bar is 50 lm. The intensity of staining for 10 individual cells from each group was determined; data are presented as means ± SD. ⁄Significant difference at p < 0.001.
120 Rneasy Fibrous Tissue Mini Kit, supplied by Qiagen, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Fig. 4. (A) Real-time RT-PCR analysis of differentially expressed GABAAa-1 gene in spinal dorsal horns (L6–S1) from rats with neonatal cystitis. Two different treatment protocols were followed. Tissues were examined <t>on</t> <t>P60</t> from the unchallenged protocol and on P30 from the re-challenged protocol. The ordinate value 2DCT corresponds to the mRNA expression relative to reference gene ribosomal <t>RNA</t> (rRNA); data are represented as mean ± SD (n = 5 per group). (B) Western blot analysis of GABAAa-1 protein in spinal dorsal horn samples (L6–S1) from rats with neonatal cystitis. The intensity of GABAAa-1 immunoreactivity for different tissues was normalized against the intensity of b-actin expression for the same tissue. Results are expressed as means ± SD (n = 5 per group). ⁄Significant difference at p < 0.05. (C) GABAAa-1 immunostaining of differentially expressed GABAAa-1 gene in spinal dorsal horns (L6–S1) from rats with neonatal cystitis. The tissues were examined on P60 after zymosan/saline treatment at P14 to P16. The scale bar is 50 lm. The intensity of staining for 10 individual cells from each group was determined; data are presented as means ± SD. ⁄Significant difference at p < 0.001.
Rneasy Fibrous Tissue Mini Rna Isolation Kit, supplied by Qiagen, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Fig. 4. (A) Real-time RT-PCR analysis of differentially expressed GABAAa-1 gene in spinal dorsal horns (L6–S1) from rats with neonatal cystitis. Two different treatment protocols were followed. Tissues were examined <t>on</t> <t>P60</t> from the unchallenged protocol and on P30 from the re-challenged protocol. The ordinate value 2DCT corresponds to the mRNA expression relative to reference gene ribosomal <t>RNA</t> (rRNA); data are represented as mean ± SD (n = 5 per group). (B) Western blot analysis of GABAAa-1 protein in spinal dorsal horn samples (L6–S1) from rats with neonatal cystitis. The intensity of GABAAa-1 immunoreactivity for different tissues was normalized against the intensity of b-actin expression for the same tissue. Results are expressed as means ± SD (n = 5 per group). ⁄Significant difference at p < 0.05. (C) GABAAa-1 immunostaining of differentially expressed GABAAa-1 gene in spinal dorsal horns (L6–S1) from rats with neonatal cystitis. The tissues were examined on P60 after zymosan/saline treatment at P14 to P16. The scale bar is 50 lm. The intensity of staining for 10 individual cells from each group was determined; data are presented as means ± SD. ⁄Significant difference at p < 0.001.
Qiaamp Fibrous Tissue Rna Kit, supplied by Qiagen, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


Fig. 4. (A) Real-time RT-PCR analysis of differentially expressed GABAAa-1 gene in spinal dorsal horns (L6–S1) from rats with neonatal cystitis. Two different treatment protocols were followed. Tissues were examined on P60 from the unchallenged protocol and on P30 from the re-challenged protocol. The ordinate value 2DCT corresponds to the mRNA expression relative to reference gene ribosomal RNA (rRNA); data are represented as mean ± SD (n = 5 per group). (B) Western blot analysis of GABAAa-1 protein in spinal dorsal horn samples (L6–S1) from rats with neonatal cystitis. The intensity of GABAAa-1 immunoreactivity for different tissues was normalized against the intensity of b-actin expression for the same tissue. Results are expressed as means ± SD (n = 5 per group). ⁄Significant difference at p < 0.05. (C) GABAAa-1 immunostaining of differentially expressed GABAAa-1 gene in spinal dorsal horns (L6–S1) from rats with neonatal cystitis. The tissues were examined on P60 after zymosan/saline treatment at P14 to P16. The scale bar is 50 lm. The intensity of staining for 10 individual cells from each group was determined; data are presented as means ± SD. ⁄Significant difference at p < 0.001.

Journal: Pain

Article Title: MicroRNA-mediated GABA Aα-1 receptor subunit down-regulation in adult spinal cord following neonatal cystitis-induced chronic visceral pain in rats.

doi: 10.1016/j.pain.2012.09.002

Figure Lengend Snippet: Fig. 4. (A) Real-time RT-PCR analysis of differentially expressed GABAAa-1 gene in spinal dorsal horns (L6–S1) from rats with neonatal cystitis. Two different treatment protocols were followed. Tissues were examined on P60 from the unchallenged protocol and on P30 from the re-challenged protocol. The ordinate value 2DCT corresponds to the mRNA expression relative to reference gene ribosomal RNA (rRNA); data are represented as mean ± SD (n = 5 per group). (B) Western blot analysis of GABAAa-1 protein in spinal dorsal horn samples (L6–S1) from rats with neonatal cystitis. The intensity of GABAAa-1 immunoreactivity for different tissues was normalized against the intensity of b-actin expression for the same tissue. Results are expressed as means ± SD (n = 5 per group). ⁄Significant difference at p < 0.05. (C) GABAAa-1 immunostaining of differentially expressed GABAAa-1 gene in spinal dorsal horns (L6–S1) from rats with neonatal cystitis. The tissues were examined on P60 after zymosan/saline treatment at P14 to P16. The scale bar is 50 lm. The intensity of staining for 10 individual cells from each group was determined; data are presented as means ± SD. ⁄Significant difference at p < 0.001.

Article Snippet: GABAAa-1 gene expression in spinal dorsal horn neurons (L6–S1) by real-time quantitative RT-PCR Total RNA was extracted from spinal dorsal horn samples from both the unchallenged (P60) and re-challenged (P30) groups of animals using Total RNA extraction kit from Bio-Rad (#732-6830).

Techniques: Quantitative RT-PCR, Expressing, Western Blot, Immunostaining, Saline, Staining